Journal: Scientific Reports
Article Title: Genetic variation, structural analysis, and virulence implications of BimA and BimC in clinical isolates of Burkholderia pseudomallei in Thailand
doi: 10.1038/s41598-024-74922-3
Figure Lengend Snippet: 3D structural models of ten BimA Bp types and BimA Bm of Australian strain MSHR668 of Burkholderia pseudomallei . ( a ) 3D structural model of BimA Bp type 1 ( B. pseudomallei K96243, classified as type 1 was used as the reference strain) as described by Stevens et al. . The yellow-colored ribbon represents the predicted signal peptide (residues 1–53); green, NIPVPPPMPGGGA direct repeat (residues 63–75); violet, proline-rich motif (residues 78–84); pink, WH and WH 2 -like domains (residues 155–158); orange, PDASX repeats (residues 244–268); blue, transmembrane domain (residues 458–516). ( b – j ) 3D structural models of BimA Bp types 2, 3, 4, 5, 6, 7, 8, 9 and 10. Mutation positions are represented in pink Corey-Pauling-Koltun (CPK) models and labeled accordingly. The red-colored ribbons represent the insertion. ( k ) 3D structural model of BimA Bm MSHR668 . All BimA Bp and BimA Bm models were built using I-TASSER. The C-score of the models were: -0.96 for BimA Bp type 1; -1.16 for type 2; -0.71 for type 3; -0.74 for type 4; -0.99 for type 5; -0.73 for type 6; -1.02 for type 7; -1.12 for type 8; -1.13 for type 9; -0.71 for type 10; and -0.93 for BimA Bm MSHR668. Figures were generated by Discovery Studio Visualizer version 21.1.
Article Snippet: The 3D structure of the model was then visualized using Discovery Studio Visualizer software (Biovia v.21.1).
Techniques: Mutagenesis, Labeling, Generated
